Isoproteins of human apolipoprotein A-II: isolation and characterization.

lsoproteins of human apolipoprotein A 4 : isolation and characterization

In human serum, polymorphism of apoA-I1 predominantly in HDLs could be demonstrated. HDLS-apoA-I1 was composed of four isoproteins, each with a molecular weight of 8600 (reduced form) and identical immunological properties. The isoproteins are designated apoA-11-1 (PI 5.16), apoA-11-2 (PI 4.89) corresponding to the already known apoAI1 monomer band, apoA-11-3 (PI 4.58), and apoA-11-4 (PI 4.31)....

THE ISOLATION OF ENZYME TRANSKETOLASE FROM HUMAN ERYTHROCYTES: THE CHARACTERIZATION OF ITS QUARTERNARY STRUCTURE

Human erythrocyte transketolase (sedoheptulose-7-phosphate: D-glyceraldehyde-3-phosphate, glycolaldehyde transferase, E.C. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 U/mg. SDS-PAGE and SE-HPLC were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. Four protein bands corresponding to molecular weights of 32,0...

The Isolation and Characterization a Human Diploid Cell Strain and Its Use in Production of Measles Vaccine

Plasma apolipoproteins in Tangier disease, as studied with two-dimensional electrophoresis.

Tangier disease is characterized by a deficiency of high-density lipoproteins and of their major protein constituent, apolipoprotein (apo) A-I. We used high-resolution two-dimensional electrophoresis to examine the principal plasma apolipoproteins (A-I, A-II, A-IV, E, C-II, and C-III) of three persons with Tangier disease, one homozygous patient and his two heterozygous children, comparing the ...

Isolation and characterization of the major plasma apolipoproteins, A-1 and B, in the European badger, Meles meles.

The two major apolipoproteins of badger serum, apoA-I and apoB, have been isolated and characterized. Apolipoprotein A-I was the principal protein of badger lipoproteins with density 1.063-1.21 g/ml and, in addition, was present in the lipoprotein class with density 1.006-1.063 g/ml. This apolipoprotein displayed an Mr of approximately equal to 27,000-28,000 and was polymorphic (three prominent...